By David M. Prescott (Ed.)
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Extra info for Methods in Cell Biology, Vol. 9
Dextran is hygroscopic. In order to make a 20% (w/w) stock solution we generally weigh out about 220 gm of dextran and make it up to 1000gm with water. The dextran slowly dissolves at room temperature with occasional shaking. After it has dissolved it is advisable to heat the solution for 15 minutes at 90-95°C to avoid subsequent microbiological contamination. Five grams of the dextran solution is weighed out and diluted 5-fold with water. Concentration of the dextran is then determined by polarimetry, [a]% = t 199", and calculated as percent (w/w).
Click, R. , and Alter, B. J. (1972). all. Immunol. 3, 156-160. Dumonde, D. C.. Wolstencroft, R. , Panayi, G. , Matthes, M.. , and Howson, W. T. (1969). Nature (London)224, 38-42. Forsdyke, D. R. (1968). Eiochem. J. 297-302. , and Harris, T. N. (1972). Proc. Exp. Eiol. Med. 140. 750-754. Greaves, M. , and Bauminger, S. (1972). Nuture (London),New Eiol. 235, 67-70. , and Peters, H. (1969). Eur. J. Eiodrem. 9, 542-549. , and Click, R. E. (1972). Cell Immunol. 5,410-418. , and Perlman, P. (1965). Nature (London) Un,818.
Ahun. Hum. Genet. 2, 259. Morton, H. J. (1970). In Ktro 6, 89. Munder, P. , and Wallach, D. F. H. (1971). FEBS (Fed. Eur. Biochem. ) Lett. 15, 191. Paul, J . (1970). “Cell and Tissue Culture,” 4th ed. Livingstone, Edinburgh. , Lorans, G . , and Beaure d’Angkres, C. (1970). Eur. J. Clin. Biol. Res. 15. 1066. van Wezel, A. L. (1967). 64. Weiss, R. , and Schleicher, G . B. (1968). Biotechnol. Bioeng. 10, 601. , Rudiger, H. , and Passarge, E. (1972). Exp. Cell Rex 74, 571. This Page Intentionally Left Blank Chapter 3 Partition of Cells in Two-Polymer Aqueous Phases : A Method for Separating Cells and for Obtaining Information on Their Surface Properties HARRY WALTER Lnboratory of Gemicnl Biology.
Methods in Cell Biology, Vol. 9 by David M. Prescott (Ed.)