By Ludwig Eichinger
Dictyostelium discoideum Protocols offers a accomplished choice of state of the art molecular organic protocols for learning D. discoideum, with emphasis on its application within the examine of basic mobile procedures together with sign transduction, chemotaxis, mobilephone motility, cytokinesis, phagocytosis, and facets of improvement comparable to telephone sorting, development formation, and mobilephone sort differentiation. Written by means of specialists within the box, this significant advisor presents easy accessibility to present equipment and strategies for the examine of D. discoideum. For the newcomer to the sphere, it deals a whole and inclusive advent to this crucial version organism. The ebook is split into 4 major elements, which come with an creation to the organism that gives crucial neighborhood assets and genome-wide methods; simple tools and to be had molecular genetic innovations for examine of the organism; imaging and localization equipment; and a dialogue of D. discoideum, with emphasis on its specific merits as a version system.
This in-depth advisor presents a whole advent to and thorough instruction manual for the research of D. discoideum, together with a dialogue of the consequences of the lately accomplished Dictyostelium genome venture in strengthening the location of D. discoideum as a version organism for learning primary phone tactics and features of improvement. Researchers in any respect degrees of expertise will enjoy the displays all through of the most up-tp-date, priceless, and leading edge recommendations for the learn of D. discoideum from prime Dictyostelium scientists.
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Extra resources for Dictyostelium discoideum Protocols
Gen. Microbiol. 99, 201–208. 9 Cox, E. , Vocke, C. , Gregg, K. , and Bain, E. S. (1990) Electro9. phoretic karyotype for Dictyostelium discoideum. Proc. Natl. Acad. Sci. USA 87, 8247–8251. The Genome of Dictyostelium discoideum 27 10 Louis, E. J. (2002) Are Drosophila telomeres an exception or the rule? Genome 10. 6. 11 Emery, H. S. and Weiner, A. M. (1981) An irregular satellite sequence is found at 11. the termini of the linear extrachromosomal rDNA in Dictyostelium discoideum. Cell 26, 411–419.
The genome of the human pathogen Entamoeba histolytica has been subjected to deep shotgun sampling and assembly into unordered scaffolds, so most of its coding capacity is known (35). These two genomes have been compared with each other and with other eukaryotic genomes in an effort to identify ameba specific properties (22). Of the 1500 orthologous gene families shared between the two amobae, most are also shared with plant, animal, and fungal genomes. Surprisingly, only 42 gene families could be defined as distinct to the ameba lineage.
2000) The mitochondrial DNA of 6. Dictyostelium discoideum: complete sequence, gene content and genome organization. Mol. Gen. Genet. 263, 514–519. 7 Robson, G. E. and Williams, K. L. (1977) The mitotic chromosomes of the cellu7. lar slime mould Dictyostelium discoideum: a karyotype based on Giemsa banding. J. Gen. Microbiol. 99, 191–200. 8 Zada-Hames, I. M. (1977) Analysis of karyotype and ploidy of Dictyostelium 8. discoideum using colchicine induced metaphase arrest. J. Gen. Microbiol. 99, 201–208.
Dictyostelium discoideum Protocols by Ludwig Eichinger