By Donald Walker
Английское пособие по самообороне середины XIX века.
Книга написана по принципу «всего по немногу».
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Example text
The protocol for construction of an RC strain is different in some ways from that for traditional congenic strains described earlier, including the number of backcrosses. 5% random contribution of the donor genome in each RC strain (108). Phenotypic characterization is followed for the relevant traits in the set of RC strains, and RC strains exhibiting phenotypic differences compared to the host strain are implicated as carrying QTLs originating from the donor strain. Rather than for fine mapping, RC strains can be more useful for the phenotype study of gene/gene interactions (109).
36. Silver LM. Laboratory Mice. , 1995:159–194. 37. O’Brien SJ, Menotti-Raymond M, Murphy WJ, Nash WG, Wienberg J, Stanyon R, Copeland NG, Jenkins NA, Womack JE, Marshall Graves JA. The promise of comparative genomics in mammals. Science 1999; 286: 458–481. 38. Love JM, Knight AM, McAleer MA, Todd JA. Towards construction of a high resolution map of the mouse genome using PCR-analyzed microsatellites. Nucl Acids Res 1990; 18:4123– 4130. 39. lakoubova OA, Olsson CL, Dains KM, Choi J, Kalcheva I, Bentley LG, Cunanan M, Hillman D, Louie J, Machrus M, West DB.
Several methods exist that can help to construct a fine map to conduct a practical search for the molecular basis of a specific QTL (21). Among those, the most commonly used strategy of isolating a QTL in the form of a congenic strain will be discussed in this review. 1. Thus, the effects of each gene can then be studied. In this simpler system, conventional genetic mapping and positional cloning methods can be applied for identification of each QTL. This strategy has proven to be highly successful in fine mapping the type I diabetes susceptibility genes, Idd3 and Idd10 (50, 51).
Defensive exercises by Donald Walker
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