Download e-book for iPad: Comprehensive Virology 11: Regulation and Genetics Plant by Lous Van Vloten-Doting, E. M. J. Jaspars (auth.), Heinz

By Lous Van Vloten-Doting, E. M. J. Jaspars (auth.), Heinz Fraenkel-Conrat, Robert R. Wagner (eds.)

ISBN-10: 1468427210

ISBN-13: 9781468427219

ISBN-10: 1468427237

ISBN-13: 9781468427233

The time turns out ripe for a severe compendium of that phase of the organic universe we name viruses. Virology, as a technological know-how, having handed only in the near past via its descriptive section of naming and num­ bering, has most likely reached that level at which rather few new­ really new-viruses can be came across. prompted via the highbrow probes and methods of molecular biology, genetics, biochemical cytology, and excessive answer microscopy and spectroscopy, the sector has skilled a real info explosion. Few critical makes an attempt were made to chronicle those occasions. This entire sequence, with the intention to include a few 6000 pages in a complete of approximately 22 volumes, represents a dedication by way of a wide staff of lively investigators to research, digest, and expostulate at the nice mass of knowledge in terms of viruses, a lot of that is now amorphous and disjointed, and scattered all through a large literature. during this means, we are hoping to put the full box in standpoint, and to advance an invalu­ capable reference and sourcebook for researchers and scholars in any respect degrees. This sequence is designed as a continuum that may be entered at any place, yet which additionally presents a logical development of constructing proof and built-in concepts.

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1 ). For other heterocapsidic viruses, this has not yet been investigated. In the mixture of RNA 4 and RNA 4a from CVV, RNA 4 is probably the active component since RNA 4a is too small to direct the synthesis of a complete coat protein molecule. , 1971) that for the initiation of an infection by the three large RNAs a small amount of coat protein is always necessary. This can be provided either as capsid, as low molecular weight coat protein, or by translation of RNA 4 in the cell. This idea is schematically represented for TSV in Fig.

U 4 2 o 10 30 slice no 50 Fig. 4. Protein synthesis in CCMV-infected protoplasts. UV-irradiated protoplasts were incubated for 24 h in the presence of actinomycin D with [3HJleucine (e, infected) or [14C]leucine (0, healthy). Drawing by courtesy of Dr. J. W. Watts. ------------. ; u ~ Fig. 5. Time course of synthesis of coat protein, peak 2 protein, and virus in UV-irradiated protoplasts after infection with CCMV. Drawing by courtesy of Dr. J. W. Watts. 2). The two bands with molecular weights of about 100,000 could represent the translation products of RNAs I and 2, respectively.

W. Davies, personal communication). It is remarkable that the translation of the large AMV RNAs was more efficient at 60°C than at 30°C. This could indicate that at this temperature the secondary structure of the RNAs has been melted. It is tempting to speculate that AMV coat protein has at 30°C a similar effect on the structure of the AMV RNAs. The BMV RNAs were translated in a cell-free system from mouse L cells and it was found that the major product resembled the authentic coat protein in electrophoretic mobility and in peptide map (Ball et at..

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Comprehensive Virology 11: Regulation and Genetics Plant Viruses by Lous Van Vloten-Doting, E. M. J. Jaspars (auth.), Heinz Fraenkel-Conrat, Robert R. Wagner (eds.)

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