By CIBA Foundation Symposium
ISBN-10: 0470514698
ISBN-13: 9780470514696
ISBN-10: 0471952346
ISBN-13: 9780471952343
Popular members offer complete insurance of calcium gradients, waves and oscillations in varied structures. Discusses the mechanisms beginning and maintaining calcium waves and their function in cellphone functionality. Describes stories utilizing the most recent options for measuring calcium ion gradients together with chemiluminescent signs.
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Extra info for Ciba Foundation Symposium 188 - Calcium Waves, Gradients and Oscillations
Sample text
Sanchez-Bueno & Cobbold (1993) showed inhibition of some hormone-induced Ca2+ oscillations in hepatocytes, and we can also see that in cells cultured for 24 h, but ryanodine itself causes waves only in freshly isolated cells. However, as Nathanson & Burgstahler (1992) showed, waves are propagated and they also have a site of origin-sometimes they have two sites of origin, which makes things more complex-but there are specific regions from which these waves propagate. In our experiments, ryanodine alone initiates Ca2+ waves with the same origin as and similar amplitude and propagation rate to those induced by agonists.
Thomas: The fura-2 signal is stable over many hours in our hands and we can come back to cells again and again and get a good fluorescence response. Cobbold: We use freshly isolated, non-attached hepatocytes, whereas you use cells maintained in culture overnight; that may be the difference. Petersen: Dr Thomas, I would like to return to your original crucial observation that the Ca2+ waves in liver cells always start from a particular location. Could you elaborate on what is special about that particular point?
Co** Content No lP3 Quanta1 Ca2+ Release 27 Subcellular organization of hepatic Ca2+ signals ER was highly fragmented, whereas the normal tubular ER structure was retained in attached cells that appeared to have continuous Ca2+ stores. Luminal continuity could also provide an explanation for the much larger size of the InsP3-sensitive pool in the attached permeabilized cells, because Ca2+ in domains of the store completely devoid of InsP3 receptors could be released via luminal Ca2 translocation.
Ciba Foundation Symposium 188 - Calcium Waves, Gradients and Oscillations by CIBA Foundation Symposium
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