By Claude Arthur Knight
In 1963, the 1st version of Chemistry of Viruses was once released as a contribution to the sequence on viruses backed by way of Protoplasmatologia. An target of the 1st variation used to be to check a few significant rules and methods of chemical virology in a concise demeanour and to accompany this evaluation with a compilation of pertinent references. It used to be expected that this workout will be worthwhile to the writer in his instructing and examine and, confidently, will be beneficial to readers besides. The literature of virology has grown vastly in view that then, and it truly is much more pressing to have a succinct survey. moreover, few authors have tried to combine the findings bearing on many of the significant sessions of viruses (that is, animal, bacterial, and plant viruses) yet, quite, have selected to collect huge monographs dealing extensive with proof and fancies touching on particular teams of viruses. Such works are worthy for pursuit of specific issues yet fail to yield a short, built-in view of virology. the current variation of Chemistry of Viruses aspires to this kind of evaluation. a major try out used to be made to deal concisely with each significant subject of chemical virology and to provide examples from varied sessions of viruses. a number of references are given to unique articles and evaluate papers in addition to to chose books.
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Additional resources for Chemistry of Viruses
H - COOH NH, CH. - CH - CH - COOH I CH. 1 TR, 1 The abbreviations are those proposed by BRAND et aL (1945) and now widely used. In peptide chains, the amino acids have lost the elements of water in fo rming peptide links to neighboring amino a cids. Such dehydratoo amino acids are Called amino acid residues, aud the abbreviations given in the table also apply to such residues when writing formulas for peptide chains (or proteins). Protopla smutologia IV, 2 3 IV, 2: C. A. 34 KNIGHT, Chelllistry of Viruses (2) Secondary siructure: ihe geometric eonfiguration of ihe peptidc chain (or chains) with special reference to the presence or absence of helica l strudure.
AIso, application of the newly developed AKABORI hydrazinolysis method (1956) confirmed tlIe original conclusion that the virus protein cOl1Jsists of many peptide chains eaeh terminating in a single threonyl resi,due (BRAUNITZER 1954: Nm aud FRAENKEL-CONRAT 1955). As shown by the equation, only the C-tcrminal amino acid comes out as the frec amino acid. AII others are eonverted to hydrazides which have different solubilities than the free amino acids and can be separated by extraction with 47 Virus Proteins appropriate organic solvents.
The supernatant is dialyzed against water to remove CaCI 2 • The dialyzed material contains the soluble protein but practically no RNA. 5. The Phenol Method (ANDERER 1959 a, Il) Disruption of viruses with phcnoi is the hasis for onc of the comlllonest methods for isolation of viral nucleic acids (sel' section on Methods for Virus Pruteins 39 Preparing Viral Nucleic Acids). In the preparation of nucleic acids the protein and other phenol-soluble components are usually discaDded in the phenolic layer.
Chemistry of Viruses by Claude Arthur Knight