By Lee E. Limbird
The objective of this booklet is to function a primer for the research of phone floor receptors. The simplified dialogue of equipment and their underlying prin ciples is meant to take away the standard intimidation brought on by the really expert vocabulary or refined arithmetic that signify some of the basic papers during this box. during this method, it really is was hoping that the elemental suggestions might be emphasised. This ebook is intended to be a kick off point: a textbook in addition to a handbook to which the investigator can go back for a refresher path, while wanted. I feit pressured to jot down this e-book for a number of purposes. the first philosophical cause was once to supply, in a single quantity, an total viewpoint at the examine ofcell floor receptors that describes their characterization from pharmacological stories in an entire organ or tissue bathtub (chapter 2) to experiences of radioligand binding in remoted membrane and detergent-solubilized pre parations (chapters 3-5) and, again back, to reviews in intact cells or tissues utilizing morphological and biochemical recommendations (chapter 6). it really is my opinion that the last word knowing of the molecular foundation for hormone or drug receptor interactions, and the physiological outcomes of those inter activities, would require an evaluate of cellphone floor receptor functionality in any respect of those experimental degrees. in lots of circumstances an investigator would possibly not decide to of research thought of here.
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Extra resources for Cell Surface Receptors: A Short Course on Theory and Methods
1927) Th e reaction between acetyl choline and muscle cells. Part 11. J. Ph ysiol. 64:123- 143. J. (1937) General Pharmacology , pp. 61-98, 176-206 and 215-217. Verlag von Jul ius Springer, Berlin. Clark. J. and Raventos, J. (1937) Th e antagonism of acetylcholine and of quar ternary arnmonium salts. Qu ant. J. Exp. Ph ysiol. 26:375- 392. Colquhoun , D . (1973) T he relation between c1assical and cooperative models for drug action . P . ), Un iversity Park, Baltimore, pp. 149- 182. H . (1914) Th e action of certain esters and ethers of choline, and their relation to mu scarine.
Despite this reservation, however, there are no reported examples of confusion of functional versus competitive antagonism having mislead investigators as to the population of receptors responsible for a given biological effect. Such a potential problem might be realized, however, in trying to resolve the receptor populations that are responsible for eliciting the multiple effects of exogenous agents, such as opiate effects in the central nervous system, where the endogenous ligand with which these receptors presumably interact has yet to be identified unequivocably.
RE PLOT A. 11 ccetrc! 0 U "'. of a"I ifreversibte receptor antoqonist 1'7; 0. 8 U ~ " l!! JE Ko. ~ i I ~ , intercept s (I-q)/q, KO A 11 (A1. un it. M" - log (A9O"istj : I AJ (A'J C. ALTERNATIVE REPLOT (A'] W . . - KOAq, K OA=(intercept- I) stcce '-in le rcepf : 1/Cl. [A') Figure 2-1. Determinatio n of the K D for recepto r-ag onist interaction s (K D A ) utilizing the techniq ue of irrev ersible receptor blockade. A . Untransform ed dat a: D ose-r espon se relation ships are determmed before (cont rol) and after expos ure of tissue preparation s to increas ing concentrations of an irreversible antago nist.
Cell Surface Receptors: A Short Course on Theory and Methods by Lee E. Limbird