Download e-book for iPad: Cell Culture for Biochemists by R.L.P. Adams (Eds.)

By R.L.P. Adams (Eds.)

ISBN-10: 0444812970

ISBN-13: 9780444812971

ISBN-10: 0444813063

ISBN-13: 9780444813060

ISBN-10: 0720442001

ISBN-13: 9780720442007

This second revised variation equals the preferred 1st variation in offering a transparent and particular evaluate of cellphone tradition. It offers details on: features of cultured cells; tradition vessels; glassware guidance and sterilisation strategies; subculturing; basic cells; phone tradition media; thoughts; illness; the mobilephone cycle; mobilephone synchronisation; use of radioactive isotopes in phone tradition; cellphone mutants and mobilephone hybrids; viruses; and differentiation in telephone cultures.

Reviews at the 1st edition:

``.. the ebook offers a very good perception into the best way cellphone tradition concepts might be hired within the analytical examine of mobile biology.'' - developments in Biochemical Sciences

``It is easily written in a concise, easy-to-read sort which stimulates the curiosity of the reader....'' - technology Tools

``A priceless guide on rules and practice.'' - Immunology at the present time

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Additional resources for Cell Culture for Biochemists

Example text

However, with a split ratio of 1 to 4 the age of the cells in generations will be twice the passage number. 3. I . Nutritional requirements In the 1940’s and early 1950’s most cells were grown in plasma or fibrinogen clots, in the presence of tissue extracts and their ultra- CH. 2. CHARACTERISTICS OF CULTURED CELLS 17 filtrates. , 1948) and the HeLa cell (Gey, 1955), were cultured on the surface of glass containers, and in a classical paper Eagle (1955) investigated their nutritional requirements.

2. CHARACTERISTICS OF CULTURED CELLS 31 proteins required for DNA synthesis and cell division (see Chapter 10). 4. Oncogenes Mutation may alter the amount or nature of a controlling growth factor or its receptor either directly or indirectly, thereby affecting the immortality of a cell. In addition, transforming viruses may introduce into recipient cells, genes (viral oncogenes) coding for proteins whch mimic growth factors or affect their production or activity. , 1983) and the src gene of Rous sarcoma virus codes for a tyrosine kinase which, like the cell surface receptors for EGF, PDGF and IGF can phosphorylate intracellular proteins involved in the transduction of the growth signal to the nucleus (Cooper and Hunter, 1981).

Gaseous exchange Gaseous exchange occurs with Petri dishes and cell culture trays and some types of suspension vessels. Tissue culture flasks often have caps which can be left in a venting position (allowing gaseous exchange) or tightly closed. When the medium used is buffered with bicarbonate (30 mM) it is essential that such cultures be maintained in an atmosphere of about 5% CO, in air. This allows maintenance of the correct pH which is readily monitored by the colour of the phenol red present in the medium.

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Cell Culture for Biochemists by R.L.P. Adams (Eds.)


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