By Awtar Krishan
A much-needed primer at the use of laser move cytometry for stem cellphone analysis
Laser circulation cytometry is a robust instrument for fast research of cells for marker expression, cellphone cycle place, proliferation, and apoptosis. despite the fact that, no assets in particular deal with using this system for the examine of stem cells; this is often specifically very important as stem telephone research contains really expert tools and marking techniques in keeping with particular features equivalent to marker expression, mobile measurement, drug delivery, and efflux of the stem cells.
Now, this e-book stories those techniques, discusses the technology at the back of them, and gives real-world examples to demonstrate the usefulness of the equipment. It brings jointly world-class specialists in pathology, biophysics, immunology, and stem mobilephone examine, who draw upon their huge event with the equipment and convey examples of fine facts to aid consultant researchers within the correct course.
bankruptcy insurance contains:
- Stem mobile research and sorting utilizing part inhabitants
- Flow cytometry within the learn of proliferation and apoptosis
- Stem mobile biology and alertness
- Identification and isolation of very small embryonic-like stem cells from murine and human specimens
- Hematopoietic stem cells—issues in enumeration
- Human embryonic stem cells: long term tradition and cardiovascular differentiation
- Limbal stem cells and corneal regeneration
- Flow cytometric sorting of spermatogonial stem cells
- Breast melanoma stem cells
- Stem phone marker expression in cells from physique hollow space fluids
This publication is a necessary source for all graduate scholars, practitioners in constructing international locations, libraries and e-book repositories of universities and study associations, and person researchers. it's also of curiosity to laboratories engaged in stem phone study and use of stem cells for tissue regeneration, and to any association dealing in stem phone and tissue regeneration research.Content:
Chapter 1 fundamentals of stream Cytometry (pages 1–12): H. Krishnamurthy and L. Scott Cram
Chapter 2 sensible issues for circulation Cytometric Sorting of Stem Cells (pages 13–23): Geoffrey W. Osborne
Chapter three Stem cellphone research and Sorting utilizing part inhabitants research (pages 25–43): William Telford
Chapter four movement Cytometry within the learn of Proliferation and Apoptosis (pages 45–60): Michael G. Ormerod and Ronald M. Hamelik
Chapter five stream Cytometric research of Drug shipping and Efflux in Stem Cells (pages 61–74): Awtar Krishan and Ronald M. Hamelik
Chapter 6 Stem cellphone Biology and alertness (pages 75–89): Swapnil Totey, Rajarshi buddy, Murali Krishna Mamidi, Vijayendran Govindasamy and Satish Totey
Chapter 7 identity and Isolation of Very SmaU Embryonic?like Stem Cells from Murine and Human Specimens (pages 91–101): Ewa okay. Zuba?Surma, Dong?Myung Shin, Habella Klich, Janina Ratajczak, Magda Kucia and Mariusz Z. Ratajczak
Chapter eight digital quantity of Hematopoietic Stem Cells (pages 103–114): Siddharth Sharma and Awtar Krishan
Chapter nine Hematopoietic Stem Cells: matters in Enumeration (pages 115–134): Michael Keeney and D. Robert Sutherland
Chapter 10 Embryonic Stem Cells: improvement and Characterization (pages 135–159): Vijay Bhaskar R. Konala, Villoo Morawala?Patell and Aparna Khanna
Chapter eleven Human Embryonic Stem Cells: Long?Term tradition and Cardiovascular Differentiation (pages 161–173): Maneesha Inamdar
Chapter 12 Mesenchymal Stromal Cells and Their scientific purposes (pages 175–188): Jyoti Kode and Vivek Tanavde
Chapter thirteen Circulating grownup Stem Cells of Hematopoietic starting place for Vascular and Neural Regeneration (pages 189–209): Lissy okay. Krishnan
Chapter 14 circulate Cytometric Characterization of Neural Progenitors Derived from Human Pluripotent Stem Cells (pages 211–222): Raj R. Rao, Sujoy okay. Dhara, Shilpa Iyer and David W. Machacek
Chapter 15 Limbal Stem Cells and Corneal Regeneration (pages 223–240): Geeta okay. Vemuganti, Murali Mohan Sagar Baila and Shubha Tiwari
Chapter sixteen movement Cytometric Sorting of Spermatogonial Stem Cells (pages 241–250): B. S. Srinag, J. M. Kalappurakkal, G.H. Mohan and H. Krishnamurthy
Chapter 17 Breast melanoma Stem Cells (pages 251–270): Devaveena Dey and Annapoorni Rangarajan
Chapter 18 Tumor Stem mobilephone Marker Expression in Cells from physique hollow space Fluids (pages 271–277): Awtar Krishan, Deepti Sharma and Ronald M. Hamelik
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Additional info for Applications of Flow Cytometry in Stem Cell Research and Tissue Regeneration
Human side population keratinocytes exhibit long-term proliferative potential and a specific gene expression profile and can form a pluristratified epidermis. Stem Cells 24:965-974. Lin KK, Goodell MA. 2006. Purification of hematopoietic stem cells using the side population. In: Methods in Enzymology, Vol. 420. Elsevier, New York, pp. 255-264. Loebinger MR, Giangreco A, Groot KR, Prichard L, Allen K, Simpson C, Bazley L, Navani N, Tibrewal S, Davies D, Janes SM. 2008. Squamous cell cancers contain a side population of stem-like cells that are made chemosensitive by ABC transporter blockade.
Pfister O, Oikonomopoulos A, Sereti KI, Sohn RL, Cullen D, Fine GC, Mouquet F, Westerman K, Liao R. 2008. Role of the ATP-binding cassette transporter Abcg2 in the phenotype and function of cardiac side population cells. Circ Res 103:825-835. Redvers RP, Li A, Kaur P. 2006. Side population in adult murine epidermis exhibits phenotypic and functional characteristics of keratinocyte stem cells. Proc Nati Acad Sei USA 103:13168-13173. Reynolds SD, Shen H, Reynolds PR, Betsuyaku T, Pilewski JM, Gambelli F, Di Giuseppe M, Ortiz LA, Stripp BR.
When murine hematopoietic cells were labeled with Hoechst dye and analyzed on a flow cytometer equipped with an ultraviolet (UV) laser, most nucleated cells labeled stoichiometrically with the dye producing a familiar G0/G1/S/G2/M cell cycle distribution. 05%) of cells showed considerably less Hoechst dye fluorescence, having effluxed the dye rapidly via a transmembrane pump. When analyzed through both a traditional ~450-nm blue Hoechst dye filter and a ~675-nm red filter and displayed in a bivariate plot, this population of cells appeared as a "tail" projecting from the normal G0/G1 population (Figure 1).
Applications of Flow Cytometry in Stem Cell Research and Tissue Regeneration by Awtar Krishan