Animal Cell Culture Methods by Jennie P. Mather and David Barnes (Eds.) PDF

By Jennie P. Mather and David Barnes (Eds.)

ISBN-10: 0124800408

ISBN-13: 9780124800403

ISBN-10: 0125441592

ISBN-13: 9780125441599

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For example, if a medium is to be optimized to achieve maximal recombinant protein secretion, then the screen should be done using the protein titer as the end point assayed. If growth is to be optimized, then the cell number is the end point. , 1990). Cells will tolerate a broad range of concentrations for some medium components but have a very narrow optimal concentration range for others. , serum, growth factor mix). For optimizing hormone and growth factor additions for serum-free culture, see Barnes and Sat0 (1980).

Many of these trace elements are normally provided as trace contaminants that enter the medium in water or serum. As the medium becomes more defined and the water more pure, these trace elements need to be purposely added to the medium formulation. Sometimes an increased concentration of vitamins can be useful. Vitamin mixtures are commercially available and can be added as such. Some vitamins, such as vitamin E (or a-tocopherol), A (retinol or retinoic acid), or C , are not added to media mixtures because of their instability, but may be important for some cells to survive or function in vitro.

1994) Many of these media are now widely used to grow many different types of cells. chapter. If end points other than cell growth are important, measure these in each of the media. Carry the cells in the medium selected for several passages and freeze them in this medium for future use. V. Screening Conditioned Medium for Biological Activity It is important to know the composition of the test medium if conditioned medium is to be screened for biological activity. As this is often done at 10-50% conditioned medium, the test medium should minimally use the same bicarbonate buffer concentration as the assay medium and optimally be identical to the assay medium except for changes introduced by the conditioning cells.

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Animal Cell Culture Methods by Jennie P. Mather and David Barnes (Eds.)

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