New PDF release: Advanced Molecular Genetics

By M. P. O’Connell, U. Priefer, N. Datta, M. E. Nugent (auth.), Alfred Pühler, Kenneth N. Timmis (eds.)

ISBN-10: 3642693059

ISBN-13: 9783642693052

ISBN-10: 3642693075

ISBN-13: 9783642693076

The improvement of strong new suggestions and refmements of tech­ niques in molecular genetics in recent times, and the surge in curiosity in biotechnology in accordance with genetic equipment, have heralded a brand new golden age in molecular genetics, and influenced in diversified disciplines a lot curiosity within the applied sciences themselves and their strength makes use of in simple and utilized biomedical sciences. even if a few very good professional laboratory manuals (especially the chilly Spring Harbor Laboratory manuals by means of I. H. Miller; R. W. Davies et al. ; and T. Maniatis et al. ) on sure chapters of molecular genetics exist, no normal textual content that covers a wide spectrum of the sub­ ject has to date been released. the aim of this guide is to pre­ despatched such a lot, even though of necessity now not all the vital tools of molecular genetics, in a sequence of straightforward experiments, lots of that are with ease entire via the microbiologist, biochemist or biotechnologist that has had in basic terms constrained publicity to genetics. the rest of the experiments require both higher familiarity with the topic, or counsel by means of a person with such adventure. The booklet may still, accordingly, not just allow members to obtain new proce­ dures for ongoing tasks, but in addition function a foundation for the instructing of molecular genetic innovations in formal predoctoral and postdoctoral laboratory courses.

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26 28 28 29 30 33 33 35 35 36 36 1. General Introduction Since the introduction of gel electrophoresis as a means of studying nucleic acids some 10 years ago, this method has become a powerful and versatile tool in the investigation and characterization of DNA molecules. It is rapid, precise, and inexpensive and requires only small amounts of DNA. Gel electrophoresis allows the rapid separation and high resolution of DNA species on the basis of molecular size and conformation.

C) Procedure . . . . . . . . . . . . . . . . d) Results. . . . . . . . . . . . . . . . . 3. Experiment 2: Mobilization of E. coli Vector Plasmids. . . . . a) Introduction. . . . . . . . . . . . . . . . b) Objectives. . . . . . . . . . . . . . . . c) Procedure . . . . . . . . . . . . . . . . d) Results. . . . . . . . . . . . . . . . . 4. Materials. . . . . . .

B) Objectives. . . . . . . . . . . . . . . . c) Procedure . . . . . . . . . . . . . . . . d) Results. . . . . . . . . . . . . . . . . 3. Experiment 2: Mobilization of E. coli Vector Plasmids. . . . . a) Introduction. . . . . . . . . . . . . . . . b) Objectives. . . . . . . . . . . . . . . . c) Procedure . . . . . . . . . . . . . . . . d) Results. . . . . . . . . .

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Advanced Molecular Genetics by M. P. O’Connell, U. Priefer, N. Datta, M. E. Nugent (auth.), Alfred Pühler, Kenneth N. Timmis (eds.)


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